Mutation Details for c.92G>T
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cDNA Name
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c.92G>T
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Protein Name
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p.Arg31Leu
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Exon or Intron
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exon 2
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Legacy Exon or Intron
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exon 2
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R31L
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Other Details
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This change has been detected by SSCP analysis of DNA amplified by PCR using the following primers: 21-5s; 5'-GTGAATATCTGTTCCTCCTC-3' and 21-3s; 5'-AGCCACCATACTTGGCTCCT-3'. The mutation can be analyzed by enzymatic digestion since the G224->T creates a new restriction site and destroys the existing one. It has been found once among 284 CF chromosomes and 144 normal chromosomes. The mutation on the other chromosome of the pancreatic sufficient CF patient is unknown.
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Contributors
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Zielinski J,
Markiewicz D,
Tsui LC
1992-10-22
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Institute
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The Hospital for Sick Children
Toronto, Canada
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Submitted Phenotype Details
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One patient, (24,female) diagnosed at 5.7 yrs, PS, normal lung function, sweat chloride 90 mEq/l.
Other mutation unknown.
(Zielenski et all 1995 and pers. corr. Zielenski)
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Reference
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Zielenski et al. 1995
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